1. Antigen: protein, peptide, organelle, cell, tissue, etc.
2 , immune methods: subcutaneous injection, intraperitoneal injection, intravenous injection, intramuscular injection .
3. The amount of antigen required for immunization of different animals (taking protein immunization as an example)
Animal antigen amount optimal antigen amount antigen amount optimal antigen amount
Rabbit 20-200 100 50-400 200
Mouse 2-40 15 4-60 40
Rat 10-50 30 20-150 50
Sheep 100-1000 200 200-1000 400
Hen 20-200 100 50-400 200
4 , immunization program (for immunized rabbits as an example):
Number of days 0 14 28 38 56 66 87
Blood collection 2ml 2ml 2+20ml 2+50-70ml
5 , pay attention to:
1) The first immunization is mixed with the immunogen with a complete adjuvant, and then the booster is mixed with the immunogen with an incomplete adjuvant, and a multi-point, time-interval immunoassay is employed.
2) If the rabbit is immunized with cells, the amount of cells required for each immunization is 2-3 x 10 7 cells.
3) After three consecutive immunizations, a small amount of blood is taken for ELISA to detect the antibody titer of the immunized animal, and the titer can reach 1:10,000-50,000. An immunization should be boosted one week before the immunization of the immunized animals.
4) If immunized with mice, a small amount of blood can be collected from the tail vein (about 50Î¼l), and finally a large amount of blood is collected from the eyeball (about 500-1000Î¼l); if immunized with rabbit, a small amount of blood can be taken from the ear vein (about 2mL), and finally Large amount of blood in the heart (50-100mL)
5) Serum was separated according to standard methods of serum preparation and dispensed in small portions and stored at -80 o C.
Related biological reagents:
Freund's complete adjuvant FCA
Superior fetal bovine serum
Premium fetal bovine serum
Signal transduction, neurological research, tumor research, etc.
Detection of human, large mouse and other series of indicators Elisa kit
Hunan Kangfutai Medical Devices Co., Ltd. , https://www.3kfut.com